diff --git a/app/test/test_cryptodev.c b/app/test/test_cryptodev.c
index d2c4c6f8b5..12e0cf8044 100644
--- a/app/test/test_cryptodev.c
+++ b/app/test/test_cryptodev.c
@@ -1371,6 +1371,42 @@ negative_hmac_sha1_testsuite_setup(void)
 	return 0;
 }
 
+static int
+negative_input_buffer_misalignment_testsuite_setup(void)
+{
+	struct crypto_testsuite_params *ts_params = &testsuite_params;
+	uint8_t dev_id = ts_params->valid_devs[0];
+	struct rte_cryptodev_info dev_info;
+	const enum rte_crypto_cipher_algorithm ciphers[] = {
+		RTE_CRYPTO_CIPHER_3DES_CBC,
+		RTE_CRYPTO_CIPHER_AES_CBC
+	};
+	const enum rte_crypto_auth_algorithm auths[] = {
+		RTE_CRYPTO_AUTH_SHA256,
+		RTE_CRYPTO_AUTH_SHA256,
+	};
+
+	rte_cryptodev_info_get(dev_id, &dev_info);
+
+	if (!(dev_info.feature_flags & RTE_CRYPTODEV_FF_SYMMETRIC_CRYPTO) ||
+			((global_api_test_type == CRYPTODEV_RAW_API_TEST) &&
+			!(dev_info.feature_flags & RTE_CRYPTODEV_FF_SYM_RAW_DP))) {
+		RTE_LOG(INFO, USER1, "Feature flag requirements for Negative "
+				"Input Buffer misalignment testsuite not met\n");
+		return TEST_SKIPPED;
+	}
+
+	if (check_cipher_capabilities_supported(ciphers, RTE_DIM(ciphers)) != 0
+			&& check_auth_capabilities_supported(auths,
+			RTE_DIM(auths)) != 0) {
+		RTE_LOG(INFO, USER1, "Capability requirements for Negative "
+				"Input Buffer misalignment testsuite not met\n");
+		return TEST_SKIPPED;
+	}
+
+	return 0;
+}
+
 static int
 dev_configure_and_start(uint64_t ff_disable)
 {
@@ -14469,6 +14505,192 @@ aes128cbc_hmac_sha1_test_vector = {
 	}
 };
 
+static const struct test_crypto_vector
+aes128cbc_sha256_misalign_test_vector = {
+	.crypto_algo = RTE_CRYPTO_CIPHER_AES_CBC,
+	.cipher_offset = 0,
+	.cipher_len = 511,
+	.cipher_key = {
+		.data = {
+			0xE4, 0x23, 0x33, 0x8A, 0x35, 0x64, 0x61, 0xE2,
+			0x49, 0x03, 0xDD, 0xC6, 0xB8, 0xCA, 0x55, 0x7A
+		},
+		.len = 16
+	},
+	.iv = {
+		.data = {
+			0x00, 0x01, 0x02, 0x03, 0x04, 0x05, 0x06, 0x07,
+			0x08, 0x09, 0x0A, 0x0B, 0x0C, 0x0D, 0x0E, 0x0F
+		},
+		.len = 16
+	},
+	.plaintext = {
+		.data = plaintext_aes_common,
+		.len = 511
+	},
+	.ciphertext = {
+		.data = ciphertext512_aes128cbc,
+		.len = 511
+	},
+	.auth_algo = RTE_CRYPTO_AUTH_SHA256,
+	.auth_offset = 0,
+	.auth_key = {
+		.data = {
+			0x42, 0x1A, 0x7D, 0x3D, 0xF5, 0x82, 0x80, 0xF1,
+			0xF1, 0x35, 0x5C, 0x3B, 0xDD, 0x9A, 0x65, 0xBA,
+			0x58, 0x34, 0x85, 0x61, 0x1C, 0x42, 0x10, 0x76,
+			0x9A, 0x4F, 0x88, 0x1B, 0xB6, 0x8F, 0xD8, 0x60
+		},
+		.len = 32
+	},
+	.digest = {
+		.data = {
+			0xA8, 0xBC, 0xDB, 0x99, 0xAA, 0x45, 0x91, 0xA3,
+			0x2D, 0x75, 0x41, 0x92, 0x28, 0x01, 0x87, 0x5D,
+			0x45, 0xED, 0x49, 0x05, 0xD3, 0xAE, 0x32, 0x57,
+			0xB7, 0x79, 0x65, 0xFC, 0xFA, 0x6C, 0xFA, 0xDF
+		},
+		.len = 32
+	}
+};
+
+static const struct test_crypto_vector
+aes192cbc_sha256_misalign_test_vector = {
+	.crypto_algo = RTE_CRYPTO_CIPHER_AES_CBC,
+	.cipher_offset = 0,
+	.cipher_len = 511,
+	.cipher_key = {
+		.data = {
+			0xE4, 0x23, 0x33, 0x8A, 0x35, 0x64, 0x61, 0xE2,
+			0x49, 0x03, 0xDD, 0xC6, 0xB8, 0xCA, 0x55, 0x7A,
+			0xD4, 0xC3, 0xA3, 0xAA, 0x33, 0x62, 0x61, 0xE0
+		},
+		.len = 24
+	},
+	.iv = {
+		.data = {
+			0x00, 0x01, 0x02, 0x03, 0x04, 0x05, 0x06, 0x07,
+			0x08, 0x09, 0x0A, 0x0B, 0x0C, 0x0D, 0x0E, 0x0F
+		},
+		.len = 16
+	},
+	.plaintext = {
+		.data = plaintext_hash,
+		.len = 511
+	},
+	.ciphertext = {
+		.data = ciphertext512_aes192cbc,
+		.len = 511
+	},
+	.auth_algo = RTE_CRYPTO_AUTH_SHA256,
+	.auth_offset = 0,
+	.auth_key = {
+		.data = {
+			0x42, 0x1A, 0x7D, 0x3D, 0xF5, 0x82, 0x80, 0xF1,
+			0xF1, 0x35, 0x5C, 0x3B, 0xDD, 0x9A, 0x65, 0xBA,
+			0x58, 0x34, 0x85, 0x61, 0x1C, 0x42, 0x10, 0x76,
+			0x9A, 0x4F, 0x88, 0x1B, 0xB6, 0x8F, 0xD8, 0x60
+		},
+		.len = 32
+	},
+	.digest = {
+		.data = {
+			0xE0, 0x4E, 0x39, 0x67, 0xB8, 0xB4, 0x56, 0xF4,
+			0x03, 0x0C, 0xF5, 0x74, 0x52, 0xFF, 0x63, 0x48,
+			0xE0, 0x3C, 0x38, 0x49, 0x76, 0x29, 0xC0, 0x57,
+			0xF4, 0x4A, 0x3E, 0x4F, 0x88, 0xB5, 0x05, 0x0E
+		},
+		.len = 32
+	}
+};
+
+static const struct test_crypto_vector
+aes256cbc_sha256_misalign_test_vector = {
+	.crypto_algo = RTE_CRYPTO_CIPHER_AES_CBC,
+	.cipher_offset = 0,
+	.cipher_len = 511,
+	.cipher_key = {
+		.data = {
+			0xE4, 0x23, 0x33, 0x8A, 0x35, 0x64, 0x61, 0xE2,
+			0x49, 0x03, 0xDD, 0xC6, 0xB8, 0xCA, 0x55, 0x7A,
+			0xD4, 0xC3, 0xA3, 0xAA, 0x33, 0x62, 0x61, 0xE0,
+			0x37, 0x07, 0xB8, 0x23, 0xA2, 0xA3, 0xB5, 0x8D
+		},
+		.len = 32
+	},
+	.iv = {
+		.data = {
+			0x00, 0x01, 0x02, 0x03, 0x04, 0x05, 0x06, 0x07,
+			0x08, 0x09, 0x0A, 0x0B, 0x0C, 0x0D, 0x0E, 0x0F
+		},
+		.len = 16
+	},
+	.plaintext = {
+		.data = plaintext_hash,
+		.len = 511
+	},
+	.ciphertext = {
+		.data = ciphertext512_aes256cbc,
+		.len = 511
+	},
+	.auth_algo = RTE_CRYPTO_AUTH_SHA256,
+	.auth_offset = 0,
+	.auth_key = {
+		.data = {
+			0x42, 0x1A, 0x7D, 0x3D, 0xF5, 0x82, 0x80, 0xF1,
+			0xF1, 0x35, 0x5C, 0x3B, 0xDD, 0x9A, 0x65, 0xBA,
+			0x58, 0x34, 0x85, 0x61, 0x1C, 0x42, 0x10, 0x76,
+			0x9A, 0x4F, 0x88, 0x1B, 0xB6, 0x8F, 0xD8, 0x60
+		},
+		.len = 32
+	},
+	.digest = {
+		.data = {
+			0x29, 0x1A, 0x38, 0x7E, 0xBC, 0x57, 0xC4, 0xB6,
+			0xD6, 0xCE, 0xEF, 0x96, 0x5D, 0x76, 0x2F, 0x3C,
+			0xDA, 0x95, 0x51, 0x82, 0xD7, 0x7B, 0x11, 0x92,
+			0x85, 0xED, 0x2D, 0xB5, 0xCF, 0x03, 0xF4, 0x54
+		},
+		.len = 32
+	}
+};
+
+static const struct test_crypto_vector
+triple_des128cbc_sha1_misalign_test_vector = {
+	.crypto_algo = RTE_CRYPTO_CIPHER_3DES_CBC,
+	.cipher_len = 511,
+	.cipher_key = {
+		.data = {
+			0xE4, 0x23, 0x33, 0x8A, 0x35, 0x64, 0x61, 0xE2,
+			0x49, 0x03, 0xDD, 0xC6, 0xB8, 0xCA, 0x55, 0x7A
+		},
+		.len = 16
+	},
+	.iv = {
+		.data = {
+			0x00, 0x01, 0x02, 0x03, 0x04, 0x05, 0x06, 0x07
+		},
+		.len = 8
+	},
+	.plaintext = {
+		.data = plaintext_hash,
+		.len = 511
+	},
+	.ciphertext = {
+		.data = ciphertext512_des128cbc,
+		.len = 511
+	},
+	.auth_algo = RTE_CRYPTO_AUTH_SHA1,
+	.digest = {
+		.data = {
+			0x94, 0x45, 0x7B, 0xDF, 0xFE, 0x80, 0xB9, 0xA6,
+			0xA0, 0x7A, 0xE8, 0x93, 0x40, 0x7B, 0x85, 0x02,
+			0x1C, 0xD7, 0xE8, 0x87
+		},
+		.len = 20
+	}
+};
+
 static const struct test_crypto_vector
 aes128cbc_hmac_sha1_aad_test_vector = {
 	.crypto_algo = RTE_CRYPTO_CIPHER_AES_CBC,
@@ -15058,7 +15280,7 @@ test_authenticated_decryption_fail_when_corruption(
 }
 
 static int
-test_authenticated_encrypt_with_esn(
+test_authenticated_encrypt(
 		struct crypto_testsuite_params *ts_params,
 		struct crypto_unittest_params *ut_params,
 		const struct test_crypto_vector *reference)
@@ -15159,7 +15381,7 @@ test_authenticated_encrypt_with_esn(
 		ut_params->op = process_crypto_request(
 			ts_params->valid_devs[0], ut_params->op);
 
-	TEST_ASSERT_NOT_NULL(ut_params->op, "no crypto operation returned");
+	TEST_ASSERT_NOT_NULL(ut_params->op, "Failed crypto process, no operation returned");
 
 	TEST_ASSERT_EQUAL(ut_params->op->status, RTE_CRYPTO_OP_STATUS_SUCCESS,
 			"crypto op processing failed");
@@ -15191,7 +15413,7 @@ test_authenticated_encrypt_with_esn(
 }
 
 static int
-test_authenticated_decrypt_with_esn(
+test_authenticated_decrypt(
 		struct crypto_testsuite_params *ts_params,
 		struct crypto_unittest_params *ut_params,
 		const struct test_crypto_vector *reference)
@@ -15291,7 +15513,7 @@ test_authenticated_decrypt_with_esn(
 		ut_params->op = process_crypto_request(ts_params->valid_devs[0],
 			ut_params->op);
 
-	TEST_ASSERT_NOT_NULL(ut_params->op, "failed crypto process");
+	TEST_ASSERT_NOT_NULL(ut_params->op, "Failed crypto process, no operation returned");
 	TEST_ASSERT_EQUAL(ut_params->op->status,
 			RTE_CRYPTO_OP_STATUS_SUCCESS,
 			"crypto op processing passed");
@@ -15850,7 +16072,7 @@ auth_decryption_AES128CBC_HMAC_SHA1_fail_tag_corrupt(void)
 static int
 auth_encrypt_AES128CBC_HMAC_SHA1_esn_check(void)
 {
-	return test_authenticated_encrypt_with_esn(
+	return test_authenticated_encrypt(
 			&testsuite_params,
 			&unittest_params,
 			&aes128cbc_hmac_sha1_aad_test_vector);
@@ -15859,12 +16081,48 @@ auth_encrypt_AES128CBC_HMAC_SHA1_esn_check(void)
 static int
 auth_decrypt_AES128CBC_HMAC_SHA1_esn_check(void)
 {
-	return test_authenticated_decrypt_with_esn(
+	return test_authenticated_decrypt(
 			&testsuite_params,
 			&unittest_params,
 			&aes128cbc_hmac_sha1_aad_test_vector);
 }
 
+static int
+test_cipher_auth_encryption_fail_data_misalignment(const void *test_data)
+{
+	struct test_crypto_vector test_vector;
+	int res;
+
+	memcpy(&test_vector, test_data, sizeof(test_vector));
+	RTE_LOG(INFO, USER1, "This is a negative test, errors are expected\n");
+	res = test_authenticated_encrypt(
+			&testsuite_params,
+			&unittest_params,
+			&test_vector);
+	if (res == TEST_SKIPPED)
+		return res;
+	TEST_ASSERT_EQUAL(res, TEST_FAILED, "encryption not failed");
+	return TEST_SUCCESS;
+}
+
+static int
+test_cipher_auth_decryption_fail_data_misalignment(const void *test_data)
+{
+	struct test_crypto_vector test_vector;
+	int res;
+
+	memcpy(&test_vector, test_data, sizeof(test_vector));
+	RTE_LOG(INFO, USER1, "This is a negative test, errors are expected\n");
+	res = test_authenticated_decrypt(
+			&testsuite_params,
+			&unittest_params,
+			&test_vector);
+	if (res == TEST_SKIPPED)
+		return res;
+	TEST_ASSERT_EQUAL(res, TEST_FAILED, "decryption not failed");
+	return TEST_SUCCESS;
+}
+
 static int
 test_chacha20_poly1305_encrypt_test_case_rfc8439(void)
 {
@@ -16675,6 +16933,56 @@ static struct unit_test_suite cryptodev_negative_hmac_sha1_testsuite = {
 	}
 };
 
+static struct unit_test_suite cryptodev_negative_input_buffer_misalignment_testsuite = {
+	.suite_name = "Negative Input buffer misalignment Unit Test Suite",
+	.setup = negative_input_buffer_misalignment_testsuite_setup,
+	.unit_test_cases = {
+		/** Negative tests */
+		TEST_CASE_NAMED_WITH_DATA(
+			"3DES128 CBC SHA1 auth encryption negative test for data misalignment",
+			ut_setup, ut_teardown,
+			test_cipher_auth_encryption_fail_data_misalignment,
+			&triple_des128cbc_sha1_misalign_test_vector),
+		TEST_CASE_NAMED_WITH_DATA(
+			"3DES128 CBC SHA1 auth decryption negative test for data misalignment",
+			ut_setup, ut_teardown,
+			test_cipher_auth_decryption_fail_data_misalignment,
+			&triple_des128cbc_sha1_misalign_test_vector),
+		TEST_CASE_NAMED_WITH_DATA(
+			"AES128 CBC SHA256 auth encryption negative test for data misalignment",
+			ut_setup, ut_teardown,
+			test_cipher_auth_encryption_fail_data_misalignment,
+			&aes128cbc_sha256_misalign_test_vector),
+		TEST_CASE_NAMED_WITH_DATA(
+			"AES128 CBC SHA256 auth decryption negative test for data misalignment",
+			ut_setup, ut_teardown,
+			test_cipher_auth_decryption_fail_data_misalignment,
+			&aes128cbc_sha256_misalign_test_vector),
+		TEST_CASE_NAMED_WITH_DATA(
+			"AES192 CBC SHA256 auth encryption negative test for data misalignment",
+			ut_setup, ut_teardown,
+			test_cipher_auth_encryption_fail_data_misalignment,
+			&aes192cbc_sha256_misalign_test_vector),
+		TEST_CASE_NAMED_WITH_DATA(
+			"AES192 CBC SHA256 auth decryption negative test for data misalignment",
+			ut_setup, ut_teardown,
+			test_cipher_auth_decryption_fail_data_misalignment,
+			&aes192cbc_sha256_misalign_test_vector),
+		TEST_CASE_NAMED_WITH_DATA(
+			"AES256 CBC SHA256 auth encryption negative test for data misalignment",
+			ut_setup, ut_teardown,
+			test_cipher_auth_encryption_fail_data_misalignment,
+			&aes256cbc_sha256_misalign_test_vector),
+		TEST_CASE_NAMED_WITH_DATA(
+			"AES256 CBC SHA256 auth decryption negative test for data misalignment",
+			ut_setup, ut_teardown,
+			test_cipher_auth_decryption_fail_data_misalignment,
+			&aes256cbc_sha256_misalign_test_vector),
+
+		TEST_CASES_END() /**< NULL terminate unit test array */
+	}
+};
+
 static struct unit_test_suite cryptodev_multi_session_testsuite = {
 	.suite_name = "Multi Session Unit Test Suite",
 	.setup = multi_session_testsuite_setup,
@@ -17567,6 +17875,7 @@ run_cryptodev_testsuite(const char *pmd_name)
 		&cryptodev_esn_testsuite,
 		&cryptodev_negative_aes_gcm_testsuite,
 		&cryptodev_negative_aes_gmac_testsuite,
+		&cryptodev_negative_input_buffer_misalignment_testsuite,
 		&cryptodev_mixed_cipher_hash_testsuite,
 		&cryptodev_negative_hmac_sha1_testsuite,
 		&cryptodev_gen_testsuite,
diff --git a/app/test/test_cryptodev_aes_test_vectors.h b/app/test/test_cryptodev_aes_test_vectors.h
index 7127156cc4..a026c2ae0e 100644
--- a/app/test/test_cryptodev_aes_test_vectors.h
+++ b/app/test/test_cryptodev_aes_test_vectors.h
@@ -1533,6 +1533,33 @@ static const struct blockcipher_test_data aes_test_data_4 = {
 	}
 };
 
+/** AES-128-CBC Negative test vector */
+static const struct blockcipher_test_data aes_128_cbc_misalign_test_data = {
+	.crypto_algo = RTE_CRYPTO_CIPHER_AES_CBC,
+	.cipher_key = {
+		.data = {
+			0xE4, 0x23, 0x33, 0x8A, 0x35, 0x64, 0x61, 0xE2,
+			0x49, 0x03, 0xDD, 0xC6, 0xB8, 0xCA, 0x55, 0x7A
+		},
+		.len = 16
+	},
+	.iv = {
+		.data = {
+			0x00, 0x01, 0x02, 0x03, 0x04, 0x05, 0x06, 0x07,
+			0x08, 0x09, 0x0A, 0x0B, 0x0C, 0x0D, 0x0E, 0x0F
+		},
+		.len = 16
+	},
+	.plaintext = {
+		.data = plaintext_aes_common,
+		.len = 511
+	},
+	.ciphertext = {
+		.data = ciphertext512_aes128cbc,
+		.len = 511
+	},
+};
+
 /** AES-128-CBC SHA1 test vector (Digest encrypted mode) */
 static const struct blockcipher_test_data aes_test_data_4_digest_enc = {
 	.crypto_algo = RTE_CRYPTO_CIPHER_AES_CBC,
@@ -2000,6 +2027,33 @@ static const struct blockcipher_test_data aes_test_data_10 = {
 	}
 };
 
+/** AES-192-CBC neg test vector */
+static const struct blockcipher_test_data aes_192_cbc_misalign_test_data = {
+	.crypto_algo = RTE_CRYPTO_CIPHER_AES_CBC,
+	.cipher_key = {
+		.data = {
+			0xE4, 0x23, 0x33, 0x8A, 0x35, 0x64, 0x61, 0xE2,
+			0x49, 0x03, 0xDD, 0xC6, 0xB8, 0xCA, 0x55, 0x7A,
+			0xD4, 0xC3, 0xA3, 0xAA, 0x33, 0x62, 0x61, 0xE0
+		},
+		.len = 24
+	},
+	.iv = {
+		.data = {
+			0x00, 0x01, 0x02, 0x03, 0x04, 0x05, 0x06, 0x07,
+			0x08, 0x09, 0x0A, 0x0B, 0x0C, 0x0D, 0x0E, 0x0F
+		},
+		.len = 16
+	},
+	.plaintext = {
+		.data = plaintext_aes_common,
+		.len = 511
+	},
+	.ciphertext = {
+		.data = ciphertext512_aes192cbc,
+		.len = 511
+	}
+};
 /** AES-192-CBC SHA1 test vector (Digest encrypted mode) */
 static const struct blockcipher_test_data aes_test_data_10_digest_enc = {
 	.crypto_algo = RTE_CRYPTO_CIPHER_AES_CBC,
@@ -2212,6 +2266,35 @@ static const struct blockcipher_test_data aes_test_data_11 = {
 	}
 };
 
+/** AES-256-CBC neg test vector */
+static const struct blockcipher_test_data aes_256_cbc_misalign_test_data = {
+	.crypto_algo = RTE_CRYPTO_CIPHER_AES_CBC,
+	.cipher_key = {
+		.data = {
+			0xE4, 0x23, 0x33, 0x8A, 0x35, 0x64, 0x61, 0xE2,
+			0x49, 0x03, 0xDD, 0xC6, 0xB8, 0xCA, 0x55, 0x7A,
+			0xD4, 0xC3, 0xA3, 0xAA, 0x33, 0x62, 0x61, 0xE0,
+			0x37, 0x07, 0xB8, 0x23, 0xA2, 0xA3, 0xB5, 0x8D
+		},
+		.len = 32
+	},
+	.iv = {
+		.data = {
+			0x00, 0x01, 0x02, 0x03, 0x04, 0x05, 0x06, 0x07,
+			0x08, 0x09, 0x0A, 0x0B, 0x0C, 0x0D, 0x0E, 0x0F
+		},
+		.len = 16
+	},
+	.plaintext = {
+		.data = plaintext_aes_common,
+		.len = 511
+	},
+	.ciphertext = {
+		.data = ciphertext512_aes256cbc,
+		.len = 511
+	}
+};
+
 /** AES-256-CBC SHA1 test vector (Digest encrypted mode) */
 static const struct blockcipher_test_data aes_test_data_11_digest_enc = {
 	.crypto_algo = RTE_CRYPTO_CIPHER_AES_CBC,
@@ -5117,6 +5200,42 @@ static const struct blockcipher_test_case aes_cipheronly_test_cases[] = {
 		.test_data = &aes_test_data_21,
 		.op_mask = BLOCKCIPHER_TEST_OP_DECRYPT,
 	},
+	{
+		.test_descr = "AES-128-CBC Encryption Negative Test for data misalignment",
+		.test_data = &aes_128_cbc_misalign_test_data,
+		.op_mask = BLOCKCIPHER_TEST_OP_ENCRYPT,
+		.feature_mask = BLOCKCIPHER_TEST_NEG_TEST,
+	},
+	{
+		.test_descr = "AES-192-CBC Encryption Negative Test for data misalignment",
+		.test_data = &aes_192_cbc_misalign_test_data,
+		.op_mask = BLOCKCIPHER_TEST_OP_ENCRYPT,
+		.feature_mask = BLOCKCIPHER_TEST_NEG_TEST,
+	},
+	{
+		.test_descr = "AES-256-CBC Encryption Negative Test for data misalignment",
+		.test_data = &aes_256_cbc_misalign_test_data,
+		.op_mask = BLOCKCIPHER_TEST_OP_ENCRYPT,
+		.feature_mask = BLOCKCIPHER_TEST_NEG_TEST,
+	},
+	{
+		.test_descr = "AES-128-CBC Decryption Negative Test for data misalignment",
+		.test_data = &aes_128_cbc_misalign_test_data,
+		.op_mask = BLOCKCIPHER_TEST_OP_DECRYPT,
+		.feature_mask = BLOCKCIPHER_TEST_NEG_TEST,
+	},
+	{
+		.test_descr = "AES-192-CBC Decryption Negative Test for data misalignment",
+		.test_data = &aes_192_cbc_misalign_test_data,
+		.op_mask = BLOCKCIPHER_TEST_OP_DECRYPT,
+		.feature_mask = BLOCKCIPHER_TEST_NEG_TEST,
+	},
+	{
+		.test_descr = "AES-256-CBC Decryption Negative Test for data misalignment",
+		.test_data = &aes_256_cbc_misalign_test_data,
+		.op_mask = BLOCKCIPHER_TEST_OP_DECRYPT,
+		.feature_mask = BLOCKCIPHER_TEST_NEG_TEST,
+	},
 };
 
 static const struct blockcipher_test_case aes_docsis_test_cases[] = {
diff --git a/app/test/test_cryptodev_blockcipher.c b/app/test/test_cryptodev_blockcipher.c
index 87a321fac3..afd5fd2f2e 100644
--- a/app/test/test_cryptodev_blockcipher.c
+++ b/app/test/test_cryptodev_blockcipher.c
@@ -100,6 +100,7 @@ test_blockcipher_one_case(const struct blockcipher_test_case *t,
 	int nb_segs_out = 1;
 	uint64_t sgl_type = t->sgl_flag;
 	uint32_t nb_iterates = 0;
+	bool validNegTestResult = false;
 
 	rte_cryptodev_info_get(dev_id, &dev_info);
 	uint64_t feat_flags = dev_info.feature_flags;
@@ -606,6 +607,8 @@ test_blockcipher_one_case(const struct blockcipher_test_case *t,
 			snprintf(test_msg, BLOCKCIPHER_TEST_MSG_LEN, "line %u "
 				"FAILED: Operation failed "
 				"(0x%X)", __LINE__, op->status);
+		if (t->feature_mask & BLOCKCIPHER_TEST_NEG_TEST)
+			validNegTestResult = true;
 		status = TEST_FAILED;
 		goto error_exit;
 	}
@@ -636,6 +639,8 @@ test_blockcipher_one_case(const struct blockcipher_test_case *t,
 				"FAILED: %s", __LINE__,
 				"Crypto data not as expected");
 			status = TEST_FAILED;
+			if (t->feature_mask & BLOCKCIPHER_TEST_NEG_TEST)
+				validNegTestResult = true;
 			goto error_exit;
 		}
 	}
@@ -655,6 +660,8 @@ test_blockcipher_one_case(const struct blockcipher_test_case *t,
 					"FAILED: %s", __LINE__, "Generated "
 					"digest data not as expected");
 				status = TEST_FAILED;
+				if (t->feature_mask & BLOCKCIPHER_TEST_NEG_TEST)
+					validNegTestResult = true;
 				goto error_exit;
 			}
 		}
@@ -806,10 +813,19 @@ test_blockcipher_one_case(const struct blockcipher_test_case *t,
 			}
 		}
 	}
-
-	snprintf(test_msg, BLOCKCIPHER_TEST_MSG_LEN, "PASS");
+	if ((status == TEST_SUCCESS) &&
+		((t->feature_mask & BLOCKCIPHER_TEST_NEG_TEST) || (validNegTestResult))) {
+		snprintf(test_msg, BLOCKCIPHER_TEST_MSG_LEN, "FAIL");
+		status = TEST_FAILED;
+	} else {
+		snprintf(test_msg, BLOCKCIPHER_TEST_MSG_LEN, "PASS");
+	}
 
 error_exit:
+	if (validNegTestResult) {
+		snprintf(test_msg, BLOCKCIPHER_TEST_MSG_LEN, "PASS");
+		status = TEST_SUCCESS;
+	}
 	if (!(t->feature_mask & BLOCKCIPHER_TEST_FEATURE_SESSIONLESS)) {
 		if (sess)
 			rte_cryptodev_sym_session_free(dev_id, sess);
diff --git a/app/test/test_cryptodev_blockcipher.h b/app/test/test_cryptodev_blockcipher.h
index e6f6c18067..b4d890d8cc 100644
--- a/app/test/test_cryptodev_blockcipher.h
+++ b/app/test/test_cryptodev_blockcipher.h
@@ -20,6 +20,7 @@
 #define BLOCKCIPHER_TEST_FEATURE_STOPPER	0x04 /* stop upon failing */
 #define BLOCKCIPHER_TEST_FEATURE_SG		0x08 /* Scatter Gather */
 #define BLOCKCIPHER_TEST_FEATURE_DIGEST_ENCRYPTED	0x10
+#define BLOCKCIPHER_TEST_NEG_TEST	0x80 /* MSB Bit is set for negative test */
 
 #define BLOCKCIPHER_TEST_OP_CIPHER	(BLOCKCIPHER_TEST_OP_ENCRYPT | \
 					BLOCKCIPHER_TEST_OP_DECRYPT)
diff --git a/app/test/test_cryptodev_des_test_vectors.h b/app/test/test_cryptodev_des_test_vectors.h
index 3ded90b6d4..9921b77501 100644
--- a/app/test/test_cryptodev_des_test_vectors.h
+++ b/app/test/test_cryptodev_des_test_vectors.h
@@ -541,6 +541,32 @@ triple_des128cbc_test_vector = {
 	}
 };
 
+static const struct blockcipher_test_data
+triple_des128cbc_misalign_test_vector = {
+	.crypto_algo = RTE_CRYPTO_CIPHER_3DES_CBC,
+	.cipher_key = {
+		.data = {
+			0xE4, 0x23, 0x33, 0x8A, 0x35, 0x64, 0x61, 0xE2,
+			0x49, 0x03, 0xDD, 0xC6, 0xB8, 0xCA, 0x55, 0x7A
+		},
+		.len = 16
+	},
+	.iv = {
+		.data = {
+			0x00, 0x01, 0x02, 0x03, 0x04, 0x05, 0x06, 0x07
+		},
+		.len = 8
+	},
+	.plaintext = {
+		.data = plaintext_des,
+		.len = 511
+	},
+	.ciphertext = {
+		.data = ciphertext512_des128cbc,
+		.len = 511
+	}
+};
+
 static const struct blockcipher_test_data
 triple_des128cbc_sha1_test_vector = {
 	.crypto_algo = RTE_CRYPTO_CIPHER_3DES_CBC,
@@ -1183,6 +1209,18 @@ static const struct blockcipher_test_case triple_des_cipheronly_test_cases[] = {
 		.test_descr = "3DES-192-CTR Decryption",
 		.test_data = &triple_des192ctr_test_vector,
 		.op_mask = BLOCKCIPHER_TEST_OP_DECRYPT,
+	},
+	{
+		.test_descr = "3DES-128-CBC Encryption Negative Test for data misalignment",
+		.test_data = &triple_des128cbc_misalign_test_vector,
+		.op_mask = BLOCKCIPHER_TEST_OP_ENCRYPT,
+		.feature_mask = BLOCKCIPHER_TEST_NEG_TEST,
+	},
+	{
+		.test_descr = "3DES-128-CBC Decryption Negative Test for data misalignment",
+		.test_data = &triple_des128cbc_misalign_test_vector,
+		.op_mask = BLOCKCIPHER_TEST_OP_DECRYPT,
+		.feature_mask = BLOCKCIPHER_TEST_NEG_TEST,
 	}
 };
 
