diff --git a/tests/TestSuite_vf_queue_start_stop.py b/tests/TestSuite_vf_queue_start_stop.py
new file mode 100644
index 00000000..bb98eb2c
--- /dev/null
+++ b/tests/TestSuite_vf_queue_start_stop.py
@@ -0,0 +1,162 @@
+# SPDX-License-Identifier: BSD-3-Clause
+# Copyright(c) 2023 Intel Corporation
+#
+
+"""
+DPDK Test suite.
+
+Test queue start stop Feature
+
+"""
+
+import os
+import re
+import time
+
+import framework.utils as utils
+from framework.packet import Packet, strip_pktload
+from framework.pmd_output import PmdOutput
+from framework.settings import FOLDERS
+from framework.test_case import TestCase
+
+#
+#
+# Test class.
+#
+
+
+class TestQueueStartStop(TestCase):
+    #
+    #
+    #
+    # Test cases.
+    #
+
+    def set_up_all(self):
+        """
+        Run at the start of each test suite.
+        """
+        self.ports = self.dut.get_ports(self.nic)
+        self.verify(len(self.ports) >= 1, "Insufficient number of ports.")
+
+        self.core_config = "1S/2C/1T"
+        self.ports_socket = self.dut.get_numa_id(self.ports[0])
+
+    def set_up(self):
+        """
+        Run before each test case.
+        """
+        # generate vf
+        self.dut.bind_interfaces_linux(self.kdriver)
+        self.dut.generate_sriov_vfs_by_port(self.ports[0], 1)
+        self.vf_port = self.dut.ports_info[self.ports[0]]["vfs_port"][0]
+
+        self.vf_port.bind_driver(driver="vfio-pci")
+        self.vf_port_pci = self.dut.ports_info[self.ports[0]]["sriov_vfs_pci"][0]
+
+    def check_forwarding(self, dmac, received=True):
+        self.send_packet(0, 0, dmac, received)
+
+    def send_packet(self, txPort, rxPort, dmac, received=True):
+        """
+        Send packages according to parameters.
+        """
+        rxitf = self.tester.get_interface(self.tester.get_local_port(rxPort))
+        txitf = self.tester.get_interface(self.tester.get_local_port(txPort))
+
+        pkt = Packet(pkt_type="UDP", pkt_len=64)
+        inst = self.tester.tcpdump_sniff_packets(rxitf)
+        pkt.config_layer("ether", {"dst": dmac})
+        pkt.send_pkt(self.tester, tx_port=txitf, count=4)
+        sniff_pkts = self.tester.load_tcpdump_sniff_packets(inst)
+
+        if received:
+            res = strip_pktload(sniff_pkts, layer="L4")
+            self.verify(
+                "58 58 58 58 58 58 58 58" in res, "receive queue not work as expected"
+            )
+        else:
+            self.verify(len(sniff_pkts) == 0, "stop queue not work as expected")
+
+    def test_vf_queue_start_stop(self):
+        """
+        vf queue start/stop test
+        """
+        self.pmd_out = PmdOutput(self.dut)
+        cores = self.dut.get_core_list(self.core_config, socket=self.ports_socket)
+        # dpdk start
+        try:
+            self.pmd_out.start_testpmd(
+                cores=cores,
+                eal_param="-a %s --file-prefix=vf" % self.vf_port_pci,
+                param="--portmask=0x1 --port-topology=loop",
+            )
+            self.dut.send_expect("set fwd mac", "testpmd>")
+            self.dut.send_expect("set verbose 1", "testpmd>")
+            self.dut.send_expect("start", "testpmd>")
+            self.pmd_out.wait_link_status_up("all")
+
+            dmac = self.pmd_out.get_port_mac(0)
+
+            self.check_forwarding(dmac)
+        except Exception as e:
+            raise IOError("dpdk start and first forward failure: %s" % e)
+
+        # stop rx queue test
+        try:
+            print("test stop rx queue")
+            self.dut.send_expect("stop", "testpmd>")
+            self.dut.send_expect("port 0 rxq 0 stop", "testpmd>")
+            self.dut.send_expect("start", "testpmd>")
+            self.check_forwarding(dmac, received=False)
+
+            # start rx queue test
+            print("test start rx queue stop tx queue")
+            self.dut.send_expect("stop", "testpmd>")
+            self.dut.send_expect("port 0 rxq 0 start", "testpmd>")
+            self.dut.send_expect("port 0 txq 0 stop", "testpmd>")
+            self.dut.send_expect("start", "testpmd>")
+            self.check_forwarding(dmac, received=False)
+            out = self.dut.get_session_output()
+        except Exception as e:
+            raise IOError("queue start/stop forward failure: %s" % e)
+        self.verify(
+            "port 0/queue 0: received 1 packets" not in out,
+            "start queue revice package failed, out = %s" % out,
+        )
+
+        try:
+            # start tx queue test
+            print("test start rx and tx queue")
+            self.dut.send_expect("stop", "testpmd>")
+            self.dut.send_expect("port 0 txq 0 start", "testpmd>")
+            self.dut.send_expect("start", "testpmd>")
+            self.check_forwarding(dmac)
+            out = self.dut.get_session_output()
+        except Exception as e:
+            raise IOError("queue start/stop forward failure: %s" % e)
+        self.verify(
+            "port 0/queue 0: received 1 packets" in out,
+            "start queue revice package failed, out = %s" % out,
+        )
+
+    def tear_down(self):
+        """
+        Run after each test case.
+        """
+        try:
+            self.dut.send_expect("stop", "testpmd>")
+            self.dut.send_expect("quit", "#")
+        except:
+            print("Failed to quit testpmd")
+
+    def tear_down_all(self):
+        """
+        Run after each test suite.
+        """
+        self.dut.kill_all()
+        self.dut.send_expect("rm -rf ./app/test-pmd/testpmd", "#")
+        self.dut.send_expect("rm -rf ./app/test-pmd/*.o", "#")
+        self.dut.send_expect("rm -rf ./app/test-pmd/build", "#")
+
+        self.dut.bind_interfaces_linux(self.drivername)
